What is Platelet-Rich Plasma (PRP)?
PRP is platelet concentrate made of autologous blood in an adequate volume of plasma.
After three decades of Platelet-Rich Plasma use in various medical fields, there is yet to be a universally-accepted definition for what PRP is.
Today there are currently number of PRP Practitioners in the world that wrongfully believe that as long as an “enrichment” sequence is performed, PRP, at any level of concentration, compared to the baseline, is attained…
What Is Effective PRP Treatment?
The known range for fragile platelet count in human blood is anywhere between 150,000 and 450,000 per microliter of Whole Blood (with an average count of 200,000/µl).
Based on recorded effective bone and soft tissue healing enhancement treatments, Medical Professionals conclude that a minimum of 1,000,000 platelet-count per microliter of PRP must be obtained in order to achieve Effective PRP. In other words, with 1,000,000 platelet count, one can achieve three to ten times the baseline. Any concentration level below three times will not be considered effective PRP; and further studies indicate that any level of platelet concentration above ten times the baseline does not offer any enhanced healing.
That said, there are three factors that influence the effectiveness of any PRP treatment:
Why Healthy Platelets?
1,000,000 fragile platelet-count per 1 microliter of PRP attains concentration levels of three to five times the baseline. In other words, to have an effective PRP treatment, the healthiness of the platelets must be well protected, because platelets are super fragile and easily become degranulated by the slightest trauma, releasing their Growth Factors before the right time, making the treatment ineffective. Therefore, one must make sure that the Growth Factors are not released until adequate platelet count is achieved.
How Fragile Platelets Become Degranulated at The Wrong Time?
Fragile platelet survival rate against any type of stress is ten to fifteen times less than the stress level that red blood cells can sustain.
There are many preventable factors that destroy fragile platelets during the PRP preparation cycle, including but not limited to, needle gauge used for blood collection, vacuum pressure in blood collection devices, transfer containers types and material, centrifugation speed, force, and duration, centrifuge machine type, and number of centrifugation cycles…
Blood-flow inside the needle is not laminar, it is turbulent. Therefore, using small gauge, long, and multiple needles for blood collection are one of the causes of fragile platelet’s breakup or degranulation.
In vacutainer blood collection devices, there are two needles. One for blood collection, and the other for collected blood’s transfer into the transfer container. In such devices, the needle gauge for blood collection is usually smaller than the needle used for transfer into transfer container. Thus, doubling the risk of fragile platelets’ degranulation.
The vacuum (PULL) pressure inside the blood collection devices such as syringe or vacutainer, where collected blood is forcefully drawn inside, can be another cause of fragile platelets’ damage, caused by the crashing of the blood cells into each other and against the hard interior surfaces of the collection devices.
In addition, there are two other types of Positive (PUSH) Pressures that fragile platelets must defy for their survival: A) the pressure caused by the force intended to push the blood inside the blood collection container out into the transfer container, which attaches and squeezes all the cells together that can cause more fragile platelets’ degranulation, and B) the pressure that is present, when the collected blood is forced through the needle into the transfer container, which in turn forces the crash of additional number of outlived fragile platelets into other blood cells and the hard interior surfaces of the transfer container.
As per American Association of Blood Banks’ recommendation, in order to protect all the blood cells, the blood collection containers must have a soft surface.
Many of the current centrifuge machine types that are used for PRP preparation are “fixed” rotor-head type. That is, during the separation cycle, containers are arranged inside the machine carriages at a fixed angle well below 180°. In other words, since the centrifugal force is parallel to the horizon at a 180°, the blood cells are propelled to the container’s back wall. The consequence of this crash is the rupture and degranulation of fragile platelets. Therefore, using a fixed rotor-head centrifuge machine cannot fully protect the fragile platelets from degranulation.
Unfortunately, many Physician and PRP Practitioners around the world are led to believe by their devices’ instruction that a single centrifugation cycle can produce the proper concentration level of platelets that are required in an effective PRP treatment.
The truth of the matter is, that although “enrichment” is possible in all PRP preparation devices, fact remains that at the end of a preparation cycle, where a single centrifugation is applied, adequate concentration level of platelets from the baseline is not obtained. Effective PRP treatment will happen when minimum three times to six times the concentration level from the baseline is attained.
If not cautious when performing a PRP treatment, considering the treatment type and specific risk factors involved with each type of treatment, the risk of infection is varied, and if not controlled can be very high, seriously endangering the wellbeing of the patient.
Note here that the risk of infection increases, each time the collected blood is transferred into another container (for example: from blood collection syringe or vacutainer into tubes, for centrifugation cycle).
EPRP™ PRP Preparation Device is a CLOSED system, which reduces the risk of cross-contamination and any possible infection risk, to none.
Have you ever asked yourself what is the right volume of PRP for any specific treatment; and how much blood must be collected from your patient in order to obtain that required volume of PRP?
Well, it is simple mathematics. Here is an example:
It is reiterated here that for successful PRP treatments, one must achieve ALL three attributes: A) High Concentration of platelets, B) Healthy, intact platelets, and C) Adequate volume of PRP.
If any of the mentioned attributes are missing, then most likely your PRP treatment will NOT be effective.
Why EPRP® PRP Preparation Device ?
How Does EPRP® Deliver Healthy Fragile Platelets?
By following National and International Scientific Organizations, Associations and Societies’ Guidelines (e.g. ISBT, AABB, WHO…) EPRP® PRP Preparation Device utilizes several methods to keep the fragile platelets healthy and undamaged:
For EPRP® PRP Preparation Device the smallest recommended Needle Gauge/Size is 19. That is, larger the needle gauge, less fragile platelets are degranulated during blood collection phase.
EPRP® PRP Preparation Device’s soft material maximizes the survival rate of fragile platelets at all times, during blood collection and separation cycles, because fragile platelets no longer crash into any hard surfaces.
In fact, AABB (American Association of Blood Banks) strongly recommends that all blood containers must be made of soft material in order to maximize the survival rate of all blood cells.
EPRP® PRP Preparation Device’s functionality is similar to blood collection procedures and guidelines being applied in every blood bank around the world; where patients’ blood is collected using natural venous pressure and actual force of gravity. There is no negative or vacuum pressure to damage the fragile platelets.
As every physician and practitioner may have had to experience how to control massive bleeding in a patient, sometimes during their careers, they would tell you that they had to inject pure, highly concentrated platelets into the patient.
For many decades, national blood banks are producing high concentration of pure platelets under industrial conditions; but today, EPRP® PRP Preparation Device’s user-friendly design helps physicians to obtain the same results, in their own private clinics.
The only difference is the level of concentration. The recommended platelet concentration levels for an effective PRP treatment is between three times to ten times the baseline. Anything below three times the baseline cannot be considered PRP; or anything above ten times the base line offers enhanced healing.
EPRP® PRP Preparation Device is created to enable the physicians and practitioners to achieve high concentration of healthy platelets at the right volumes of PRP, in their own private practices.
To achieve 1,000,000/µl fragile platelet count, we need to delicately separate RBCs and WBCs from fragile platelets, contained within the plasma. This is attained by the first centrifugation cycle.
Obviously, a small percentage of fragile platelets cannot make the journey because they are held back by the bigger RBCs.
Don’t forget that at the end of the first centrifugation cycle, the concentration level of platelets is less than twice the baseline. That is exactly why AABB recommends double-centrifugation procedure, because this is the only way that fragile platelets will separate from plasma, and minimum three times the base line concentration of platelets can be achieved.
Therefore, the right PRP concentration is obtained only after the second spin cycle, where fragile platelets are separated from Platelet-Poor Plasma (PPP); resulting in the highest collectable platelet count.
Medical Fields That Can Highly Benefit From EPRP®
In some critical medical situations, where taking more blood would possibly jeopardize patients’ life (e.g. burn victims), and loss of more blood is not an option; while high concentration of platelets are required to aid in speeding the healing process, only EPRP® PRP Preparation Device makes it possible to re-transfuse patient’s own blood (packed cells and plasma) back into the patient’s own body.
This priceless feature is also advantageous for huge Lipotransfer operations, where initially patient loses excessive volume of blood, during the fat harvesting procedure; where at the completion of PRP preparation procedure, the packed cells and Poor-Platelet Plasma can be re-transfused into patient.
In the Triple-Bag Preparation Device, the transferred PPP (Platelet-Poor Plasma) to the third bag, can be used to produce Bio Filler / Plasma Filler for the same patient.
The PPP can also be used to produce moisturizing cream for the same patient.
EPRP® PRP Preparation Device is the most cost-beneficial, value-for-money, PRP preparation device available in the market. It is offered in three convenient blood collection volume sizes:
With one blood collection, patient can be treated to several PRP treatments in one session.
The result of effective PRP treatment is the stimulation of fibroblast growth factors for production of collagens (known as Collagen Induction Therapy (CIT)); the effect for which will be apparent to the patient only after few months of multiple treatments.
Unfortunately, there is a common misinterpretation that exists among Aesthetic Physicians about PRP treatment in skin rejuvenation therapy verses simple plasma injection.
Many physicians confuse the quick effect of “skin hydration” that is due to the injection of plasma (which contains proteins, electrolytes and many other components, that trigger the skin’s natural moisture to charge to the surface of the skin via osmotic pressure, giving the skin a temporary, healthy-looking, reduced-wrinkles, glowing effect); as the indication of the effectiveness of their PRP Treatment.
Again, the true intention for applying PRP treatment for Skin Rejuvenation is to induce cell growth in the skin, which results in retarding and reversing the aging process, also known as Collagen Induction Therapy (CIT); which takes few months of multiple treatment sessions.
ARA International Enterprises
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